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mpo inhibitor
Mpo Inhibitor, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mpo+inhibitor/4-Aminobenzoic+acid/pm40598534-81-22-28
Average 93 stars, based on 1 article reviews
Mpo Inhibitor, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mpo+inhibitor/4-Aminobenzoic+acid/pm40598534-81-22-28
Average 93 stars, based on 1 article reviews
mpo inhibitor - by Bioz Stars,
2026-09
93/100 stars
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1) Product Images from "Pentatrichomonas hominis induces extracellular traps formation of macrophages via the TLR2/NADPH/PAD4 pathway."
Article Title: Pentatrichomonas hominis induces extracellular traps formation of macrophages via the TLR2/NADPH/PAD4 pathway.
Journal: Parasites & vectors
doi: 10.1186/s13071-025-06840-w
Figure Legend Snippet: Fig. 5 NADPH activation and ROS production were involved in the formation of P. hominis-induced METs. a, Macrophages (7.5 × 105 cells/ml) were pretreated with NADPH oxidase inhibitor (DPI) prior to P. hominis trophozoite stimulation for 30 min. b, ROS production was detected via DCFH-DA. Macrophages were pretreated or untreated with DPI. The macrophages stimulated with zymosan (1 mg/ml) were used as a positive control, and unstimulated macrophages were used as a negative control. c, Macrophages (7.5 × 105 cells/ml) were pretreated with MPO inhibitor (4-ABAH) prior to P. hominis trophozoite stimulation for 30 min. d, DNase I was added to the coincubated medium 15 min before the end of incubation. The dsDNA produced by METs in supernatants was stained with PicoGreen and detected. Bars represent the mean ± SD for the three experiments, ***P < 0.001, ****P < 0.0001
Techniques Used: Activation Assay, Positive Control, Negative Control, Incubation, Produced, Staining
Related Articles
Activation Assay:Article Title: Pentatrichomonas hominis induces extracellular traps formation of macrophages via the TLR2/NADPH/PAD4 pathway. Article Snippet: The cells incubated with 1 mg/ml zymosan (different time grouping: 30, 60, 90, 120, 150, and 180 min; different ratios grouping: 120 min) or culture medium were used as positive and negative controls, respectively.. In parallel experiments, macrophages were pretreated with 20 μM of NADPH oxidase inhibitor (diphenylene iodonium [DPI], Merck, D2926, USA), Article Title: Pentatrichomonas hominis induces extracellular traps formation of macrophages via the TLR2/NADPH/PAD4 pathway Article Snippet: The cells incubated with 1 mg/ml zymosan (different time grouping: 30, 60, 90, 120, 150, and 180 min; different ratios grouping: 120 min) or culture medium were used as positive and negative controls, respectively.. 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TECs were treated in the presence or absence of NETs, degranulation substrate, DNase I (2 µg/mL, Sigma), an MMP9 inhibitor (Sivelestat, 10 µM, Selleck), an NE inhibitor (SB-3CT, 10 µM, Selleck), an Positive Control:Article Title: Pentatrichomonas hominis induces extracellular traps formation of macrophages via the TLR2/NADPH/PAD4 pathway. Article Snippet: The cells incubated with 1 mg/ml zymosan (different time grouping: 30, 60, 90, 120, 150, and 180 min; different ratios grouping: 120 min) or culture medium were used as positive and negative controls, respectively.. 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In parallel experiments, macrophages were pretreated with 20 μM of NADPH oxidase inhibitor (diphenylene iodonium [DPI], Merck, D2926, USA), Article Title: Pentatrichomonas hominis induces extracellular traps formation of macrophages via the TLR2/NADPH/PAD4 pathway Article Snippet: The cells incubated with 1 mg/ml zymosan (different time grouping: 30, 60, 90, 120, 150, and 180 min; different ratios grouping: 120 min) or culture medium were used as positive and negative controls, respectively.. In parallel experiments, macrophages were pretreated with 20 μM of NADPH oxidase inhibitor (diphenylene iodonium [DPI], Merck, D2926, USA), Article Title: Neutrophil extracellular trap-derived double-stranded RNA aggravates PANoptosis in renal ischemia reperfusion injury. Article Snippet: Briefly, TECs were cultured in glucose-free DMEM (Gibco) and exposed to 95% N2 and 5% CO2 for 12 h. 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In parallel experiments, macrophages were pretreated with 20 μM of NADPH oxidase inhibitor (diphenylene iodonium [DPI], Merck, D2926, USA), Article Title: Pentatrichomonas hominis induces extracellular traps formation of macrophages via the TLR2/NADPH/PAD4 pathway Article Snippet: The cells incubated with 1 mg/ml zymosan (different time grouping: 30, 60, 90, 120, 150, and 180 min; different ratios grouping: 120 min) or culture medium were used as positive and negative controls, respectively.. In parallel experiments, macrophages were pretreated with 20 μM of NADPH oxidase inhibitor (diphenylene iodonium [DPI], Merck, D2926, USA), Article Title: Neutrophil extracellular trap-derived double-stranded RNA aggravates PANoptosis in renal ischemia reperfusion injury. Article Snippet: Briefly, TECs were cultured in glucose-free DMEM (Gibco) and exposed to 95% N2 and 5% CO2 for 12 h. Then, the glucose-free DMEM was replaced with normal DMEM, and the cells were returned to a normoxic chamber for 2 h of reoxygenation.Briefly, TECs were cultured in glucose-free DMEM (Gibco) and exposed to 95% N2 and 5% CO2 for 12 h. Then, the glucose-free DMEM was replaced with normal DMEM, and the cells were returned to a normoxic chamber for 2 h of reoxygenation.. TECs were treated in the presence or absence of NETs, degranulation substrate, DNase I (2 μg/mL, Sigma), an MMP9 inhibitor (Sivelestat, 10 μM, Selleck), an NE inhibitor (SB-3CT, 10 μM, Selleck), an MPO Article Title: Neutrophil extracellular trap-derived double-stranded RNA aggravates PANoptosis in renal ischemia reperfusion injury Article Snippet: Briefly, TECs were cultured in glucose-free DMEM (Gibco) and exposed to 95% N 2 and 5% CO 2 for 12 h. Then, the glucose-free DMEM was replaced with normal DMEM, and the cells were returned to a normoxic chamber for 2 h of reoxygenation.TECs were cultured in glucose-free DMEM (Gibco) and exposed to 95% N 2 and 5% CO 2 for 12 h. Then, the glucose-free DMEM was replaced with normal DMEM, and the cells were returned to a normoxic chamber for 2 h of reoxygenation. ... TECs were treated in the presence or absence of NETs, degranulation substrate, DNase I (2 µg/mL, Sigma), an MMP9 inhibitor (Sivelestat, 10 µM, Selleck), an NE inhibitor (SB-3CT, 10 µM, Selleck), an Staining:Article Title: Pentatrichomonas hominis induces extracellular traps formation of macrophages via the TLR2/NADPH/PAD4 pathway. Article Snippet: The cells incubated with 1 mg/ml zymosan (different time grouping: 30, 60, 90, 120, 150, and 180 min; different ratios grouping: 120 min) or culture medium were used as positive and negative controls, respectively.. In parallel experiments, macrophages were pretreated with 20 μM of NADPH oxidase inhibitor (diphenylene iodonium [DPI], Merck, D2926, USA), Article Title: Pentatrichomonas hominis induces extracellular traps formation of macrophages via the TLR2/NADPH/PAD4 pathway Article Snippet: The cells incubated with 1 mg/ml zymosan (different time grouping: 30, 60, 90, 120, 150, and 180 min; different ratios grouping: 120 min) or culture medium were used as positive and negative controls, respectively.. In parallel experiments, macrophages were pretreated with 20 μM of NADPH oxidase inhibitor (diphenylene iodonium [DPI], Merck, D2926, USA), Article Title: Neutrophil extracellular trap-derived double-stranded RNA aggravates PANoptosis in renal ischemia reperfusion injury. Article Snippet: Briefly, TECs were cultured in glucose-free DMEM (Gibco) and exposed to 95% N2 and 5% CO2 for 12 h. 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Then, the glucose-free DMEM was replaced with normal DMEM, and the cells were returned to a normoxic chamber for 2 h of reoxygenation.TECs were cultured in glucose-free DMEM (Gibco) and exposed to 95% N 2 and 5% CO 2 for 12 h. Then, the glucose-free DMEM was replaced with normal DMEM, and the cells were returned to a normoxic chamber for 2 h of reoxygenation. ... TECs were treated in the presence or absence of NETs, degranulation substrate, DNase I (2 µg/mL, Sigma), an MMP9 inhibitor (Sivelestat, 10 µM, Selleck), an NE inhibitor (SB-3CT, 10 µM, Selleck), an Derivative Assay:Article Title: Pentatrichomonas hominis induces extracellular traps formation of macrophages via the TLR2/NADPH/PAD4 pathway. Article Snippet: The cells incubated with 1 mg/ml zymosan (different time grouping: 30, 60, 90, 120, 150, and 180 min; different ratios grouping: 120 min) or culture medium were used as positive and negative controls, respectively.. In parallel experiments, macrophages were pretreated with 20 μM of NADPH oxidase inhibitor (diphenylene iodonium [DPI], Merck, D2926, USA), Article Title: Pentatrichomonas hominis induces extracellular traps formation of macrophages via the TLR2/NADPH/PAD4 pathway Article Snippet: The cells incubated with 1 mg/ml zymosan (different time grouping: 30, 60, 90, 120, 150, and 180 min; different ratios grouping: 120 min) or culture medium were used as positive and negative controls, respectively.. In parallel experiments, macrophages were pretreated with 20 μM of NADPH oxidase inhibitor (diphenylene iodonium [DPI], Merck, D2926, USA), Article Title: Neutrophil extracellular trap-derived double-stranded RNA aggravates PANoptosis in renal ischemia reperfusion injury. Article Snippet: Briefly, TECs were cultured in glucose-free DMEM (Gibco) and exposed to 95% N2 and 5% CO2 for 12 h. Then, the glucose-free DMEM was replaced with normal DMEM, and the cells were returned to a normoxic chamber for 2 h of reoxygenation.Briefly, TECs were cultured in glucose-free DMEM (Gibco) and exposed to 95% N2 and 5% CO2 for 12 h. Then, the glucose-free DMEM was replaced with normal DMEM, and the cells were returned to a normoxic chamber for 2 h of reoxygenation.. TECs were treated in the presence or absence of NETs, degranulation substrate, DNase I (2 μg/mL, Sigma), an MMP9 inhibitor (Sivelestat, 10 μM, Selleck), an NE inhibitor (SB-3CT, 10 μM, Selleck), an MPO Article Title: Neutrophil extracellular trap-derived double-stranded RNA aggravates PANoptosis in renal ischemia reperfusion injury Article Snippet: Briefly, TECs were cultured in glucose-free DMEM (Gibco) and exposed to 95% N 2 and 5% CO 2 for 12 h. Then, the glucose-free DMEM was replaced with normal DMEM, and the cells were returned to a normoxic chamber for 2 h of reoxygenation.TECs were cultured in glucose-free DMEM (Gibco) and exposed to 95% N 2 and 5% CO 2 for 12 h. Then, the glucose-free DMEM was replaced with normal DMEM, and the cells were returned to a normoxic chamber for 2 h of reoxygenation. ... TECs were treated in the presence or absence of NETs, degranulation substrate, DNase I (2 µg/mL, Sigma), an MMP9 inhibitor (Sivelestat, 10 µM, Selleck), an NE inhibitor (SB-3CT, 10 µM, Selleck), an Western Blot:Article Title: Pentatrichomonas hominis induces extracellular traps formation of macrophages via the TLR2/NADPH/PAD4 pathway. Article Snippet: The cells incubated with 1 mg/ml zymosan (different time grouping: 30, 60, 90, 120, 150, and 180 min; different ratios grouping: 120 min) or culture medium were used as positive and negative controls, respectively.. In parallel experiments, macrophages were pretreated with 20 μM of NADPH oxidase inhibitor (diphenylene iodonium [DPI], Merck, D2926, USA), Article Title: Pentatrichomonas hominis induces extracellular traps formation of macrophages via the TLR2/NADPH/PAD4 pathway Article Snippet: The cells incubated with 1 mg/ml zymosan (different time grouping: 30, 60, 90, 120, 150, and 180 min; different ratios grouping: 120 min) or culture medium were used as positive and negative controls, respectively.. In parallel experiments, macrophages were pretreated with 20 μM of NADPH oxidase inhibitor (diphenylene iodonium [DPI], Merck, D2926, USA), Article Title: Neutrophil extracellular trap-derived double-stranded RNA aggravates PANoptosis in renal ischemia reperfusion injury. Article Snippet: Briefly, TECs were cultured in glucose-free DMEM (Gibco) and exposed to 95% N2 and 5% CO2 for 12 h. Then, the glucose-free DMEM was replaced with normal DMEM, and the cells were returned to a normoxic chamber for 2 h of reoxygenation.Briefly, TECs were cultured in glucose-free DMEM (Gibco) and exposed to 95% N2 and 5% CO2 for 12 h. Then, the glucose-free DMEM was replaced with normal DMEM, and the cells were returned to a normoxic chamber for 2 h of reoxygenation.. TECs were treated in the presence or absence of NETs, degranulation substrate, DNase I (2 μg/mL, Sigma), an MMP9 inhibitor (Sivelestat, 10 μM, Selleck), an NE inhibitor (SB-3CT, 10 μM, Selleck), an MPO Article Title: Neutrophil extracellular trap-derived double-stranded RNA aggravates PANoptosis in renal ischemia reperfusion injury Article Snippet: Briefly, TECs were cultured in glucose-free DMEM (Gibco) and exposed to 95% N 2 and 5% CO 2 for 12 h. 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In parallel experiments, macrophages were pretreated with 20 μM of NADPH oxidase inhibitor (diphenylene iodonium [DPI], Merck, D2926, USA), Article Title: Pentatrichomonas hominis induces extracellular traps formation of macrophages via the TLR2/NADPH/PAD4 pathway Article Snippet: The cells incubated with 1 mg/ml zymosan (different time grouping: 30, 60, 90, 120, 150, and 180 min; different ratios grouping: 120 min) or culture medium were used as positive and negative controls, respectively.. In parallel experiments, macrophages were pretreated with 20 μM of NADPH oxidase inhibitor (diphenylene iodonium [DPI], Merck, D2926, USA), Article Title: Neutrophil extracellular trap-derived double-stranded RNA aggravates PANoptosis in renal ischemia reperfusion injury. Article Snippet: Briefly, TECs were cultured in glucose-free DMEM (Gibco) and exposed to 95% N2 and 5% CO2 for 12 h. Then, the glucose-free DMEM was replaced with normal DMEM, and the cells were returned to a normoxic chamber for 2 h of reoxygenation.Briefly, TECs were cultured in glucose-free DMEM (Gibco) and exposed to 95% N2 and 5% CO2 for 12 h. Then, the glucose-free DMEM was replaced with normal DMEM, and the cells were returned to a normoxic chamber for 2 h of reoxygenation.. TECs were treated in the presence or absence of NETs, degranulation substrate, DNase I (2 μg/mL, Sigma), an MMP9 inhibitor (Sivelestat, 10 μM, Selleck), an NE inhibitor (SB-3CT, 10 μM, Selleck), an MPO Article Title: Neutrophil extracellular trap-derived double-stranded RNA aggravates PANoptosis in renal ischemia reperfusion injury Article Snippet: Briefly, TECs were cultured in glucose-free DMEM (Gibco) and exposed to 95% N 2 and 5% CO 2 for 12 h. Then, the glucose-free DMEM was replaced with normal DMEM, and the cells were returned to a normoxic chamber for 2 h of reoxygenation.TECs were cultured in glucose-free DMEM (Gibco) and exposed to 95% N 2 and 5% CO 2 for 12 h. Then, the glucose-free DMEM was replaced with normal DMEM, and the cells were returned to a normoxic chamber for 2 h of reoxygenation. ... TECs were treated in the presence or absence of NETs, degranulation substrate, DNase I (2 µg/mL, Sigma), an MMP9 inhibitor (Sivelestat, 10 µM, Selleck), an NE inhibitor (SB-3CT, 10 µM, Selleck), an Confocal Microscopy:Article Title: Pentatrichomonas hominis induces extracellular traps formation of macrophages via the TLR2/NADPH/PAD4 pathway. Article Snippet: The cells incubated with 1 mg/ml zymosan (different time grouping: 30, 60, 90, 120, 150, and 180 min; different ratios grouping: 120 min) or culture medium were used as positive and negative controls, respectively.. In parallel experiments, macrophages were pretreated with 20 μM of NADPH oxidase inhibitor (diphenylene iodonium [DPI], Merck, D2926, USA), Article Title: Pentatrichomonas hominis induces extracellular traps formation of macrophages via the TLR2/NADPH/PAD4 pathway Article Snippet: The cells incubated with 1 mg/ml zymosan (different time grouping: 30, 60, 90, 120, 150, and 180 min; different ratios grouping: 120 min) or culture medium were used as positive and negative controls, respectively.. In parallel experiments, macrophages were pretreated with 20 μM of NADPH oxidase inhibitor (diphenylene iodonium [DPI], Merck, D2926, USA), Article Title: Neutrophil extracellular trap-derived double-stranded RNA aggravates PANoptosis in renal ischemia reperfusion injury. Article Snippet: Briefly, TECs were cultured in glucose-free DMEM (Gibco) and exposed to 95% N2 and 5% CO2 for 12 h. Then, the glucose-free DMEM was replaced with normal DMEM, and the cells were returned to a normoxic chamber for 2 h of reoxygenation.Briefly, TECs were cultured in glucose-free DMEM (Gibco) and exposed to 95% N2 and 5% CO2 for 12 h. Then, the glucose-free DMEM was replaced with normal DMEM, and the cells were returned to a normoxic chamber for 2 h of reoxygenation.. TECs were treated in the presence or absence of NETs, degranulation substrate, DNase I (2 μg/mL, Sigma), an MMP9 inhibitor (Sivelestat, 10 μM, Selleck), an NE inhibitor (SB-3CT, 10 μM, Selleck), an MPO Article Title: Neutrophil extracellular trap-derived double-stranded RNA aggravates PANoptosis in renal ischemia reperfusion injury Article Snippet: Briefly, TECs were cultured in glucose-free DMEM (Gibco) and exposed to 95% N 2 and 5% CO 2 for 12 h. Then, the glucose-free DMEM was replaced with normal DMEM, and the cells were returned to a normoxic chamber for 2 h of reoxygenation.TECs were cultured in glucose-free DMEM (Gibco) and exposed to 95% N 2 and 5% CO 2 for 12 h. Then, the glucose-free DMEM was replaced with normal DMEM, and the cells were returned to a normoxic chamber for 2 h of reoxygenation. ... TECs were treated in the presence or absence of NETs, degranulation substrate, DNase I (2 µg/mL, Sigma), an MMP9 inhibitor (Sivelestat, 10 µM, Selleck), an NE inhibitor (SB-3CT, 10 µM, Selleck), an Flow Cytometry:Article Title: Pentatrichomonas hominis induces extracellular traps formation of macrophages via the TLR2/NADPH/PAD4 pathway. Article Snippet: The cells incubated with 1 mg/ml zymosan (different time grouping: 30, 60, 90, 120, 150, and 180 min; different ratios grouping: 120 min) or culture medium were used as positive and negative controls, respectively.. In parallel experiments, macrophages were pretreated with 20 μM of NADPH oxidase inhibitor (diphenylene iodonium [DPI], Merck, D2926, USA), Article Title: Pentatrichomonas hominis induces extracellular traps formation of macrophages via the TLR2/NADPH/PAD4 pathway Article Snippet: The cells incubated with 1 mg/ml zymosan (different time grouping: 30, 60, 90, 120, 150, and 180 min; different ratios grouping: 120 min) or culture medium were used as positive and negative controls, respectively.. In parallel experiments, macrophages were pretreated with 20 μM of NADPH oxidase inhibitor (diphenylene iodonium [DPI], Merck, D2926, USA), Article Title: Neutrophil extracellular trap-derived double-stranded RNA aggravates PANoptosis in renal ischemia reperfusion injury. Article Snippet: Briefly, TECs were cultured in glucose-free DMEM (Gibco) and exposed to 95% N2 and 5% CO2 for 12 h. Then, the glucose-free DMEM was replaced with normal DMEM, and the cells were returned to a normoxic chamber for 2 h of reoxygenation.Briefly, TECs were cultured in glucose-free DMEM (Gibco) and exposed to 95% N2 and 5% CO2 for 12 h. Then, the glucose-free DMEM was replaced with normal DMEM, and the cells were returned to a normoxic chamber for 2 h of reoxygenation.. TECs were treated in the presence or absence of NETs, degranulation substrate, DNase I (2 μg/mL, Sigma), an MMP9 inhibitor (Sivelestat, 10 μM, Selleck), an NE inhibitor (SB-3CT, 10 μM, Selleck), an MPO Article Title: Neutrophil extracellular trap-derived double-stranded RNA aggravates PANoptosis in renal ischemia reperfusion injury Article Snippet: Briefly, TECs were cultured in glucose-free DMEM (Gibco) and exposed to 95% N 2 and 5% CO 2 for 12 h. Then, the glucose-free DMEM was replaced with normal DMEM, and the cells were returned to a normoxic chamber for 2 h of reoxygenation.TECs were cultured in glucose-free DMEM (Gibco) and exposed to 95% N 2 and 5% CO 2 for 12 h. Then, the glucose-free DMEM was replaced with normal DMEM, and the cells were returned to a normoxic chamber for 2 h of reoxygenation. ... TECs were treated in the presence or absence of NETs, degranulation substrate, DNase I (2 µg/mL, Sigma), an MMP9 inhibitor (Sivelestat, 10 µM, Selleck), an NE inhibitor (SB-3CT, 10 µM, Selleck), an |